Saliva as a biomarker of heat shock protein 70 in poor oral health

Background: The Heat shock proteins are stress induced and there is increased evidence of presence of these chaperons extracellular which is considered to be intracellular. Their present cytokine like effect and help in immune recognition, thus indicate strong extracellular action of heat shock protein 70 in maintaining integrity of oral cavity. Hypothesis: The aim of this study was to evaluate the Circulatory and salivary heat shock protein level 70 in healthy individuals and individuals with poor oral health. Evaluation: Settings and Design: 80 patients attending to the outpatient department of A.B. Shetty memorial institute of dental sciences, Mangalore during the period of October 2015 to November 2015 were included in the study. Control group (N=40) includes healthy individuals with good oral hygiene (OHIS SCORE > 3) and Experimental group (N=40) includes individuals with poor oral hygiene (OHIS< 3). Methods and Material: Saliva and serum samples were evaluated for Heat shock protein 70 levels in test and control group by ELISA kit method. Statistical analysis used: Student’s t test was used for statistical analysis, which was expressed in terms of mean and standard deviation. P<0.05 was considered to be statistically significant. Results: There was a significant increase in Serum Heat shock protein 70 levels in Experimental group (5.87 ng/ml) in comparison with the control group (3.170ng/ml). Salivary Heat shock protein 70 shock showed significant levels in experimental levels (4.21 ng/dl) compared to control group (2.641ng/dl) Conclusions: Heat shock proteins 70 are efficient cell stress marker in poor oral health condition